Journal: Cell Metabolism

Article Title: Mosaic dysfunction of mitophagy in mitochondrial muscle disease

doi: 10.1016/j.cmet.2021.12.017

Figure Lengend Snippet:

Article Snippet: VECTASTAIN Elite ABC-Peroxidase Kit , Vector Laboratories , Cat# PK-6101; RRID: AB_2336820.

Techniques: Plasmid Preparation, Recombinant, Avidin-Biotin Assay, Blocking Assay, Software, Microscopy, Laser-Scanning Microscopy

Journal: Journal of Biological Chemistry

Article Title: Cartilage Oligomeric Matrix Protein Associates with Granulin-Epithelin Precursor (GEP) and Potentiates GEP-stimulated Chondrocyte Proliferation

doi: 10.1074/jbc.m608744200

Figure Lengend Snippet: FIGURE 6. Immunohistochemistry of COMP and GEP in the section of long bone from a 19-day-old mouse embryo. A, low power microphotograph of a section stained with anti-GEP polyclonal antibody (red) and counterstained with Mayer’s hematoxylin (blue). Immunostaining for GEP demonstrates localization of strongly immunopositive chondrocytes in the lower prolifera- tive/upper hypertrophic zones of the growth plate. B, high power micropho- tograph of section in A. C, low power microphotograph of section stained with anti-COMP polyclonal antibody (red) and counterstained with Mayer’s hematoxylin (blue); immunostaining reveals positive staining in chondro- cytes. D, high power microphotograph of section in C. S, resting chondro- cytes; P, proliferating chondrocytes; H, hypertrophic chondrocytes; M, bone metaphysis. Bar, 100 m.

Article Snippet: After rehydration in phosphate-buffered saline and blocking with 20% goat serum in phosphate-buffered saline for 30 min, the cells were incubated with primary antibodies (i.e. mouse monoclonal anti-COMP antibodies (diluted 1:50) and polyclonal goat anti-GEP antibodies (Santa Cruz Biotechnology; diluted 1:50) at room temperature for 1 h.After beingwashedwith phosphate-buffered saline, the coverslips were incubated with secondary antibodies (i.e. goat anti-mouse IgG conjugated with rhodamine (Santa Cruz Biotechnology; diluted 1:100) and chick anti-goat IgG conjugated with fluorescein isothiocyanate (Santa Cruz Biotechnology; diluted 1:400) for 50 min.

Techniques: Immunohistochemistry, Staining, Immunostaining